Prep HPLC Column, Bidentate C8, 4um, 100A. 30mm ID x 50mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40008‑P30‑50 Cogent Bidentate C8 Preparative Column is designed for high‑capacity purification and preparative HPLC separations, using 4 µm, 100 Å TYPE‑C™ silica hydride particles to deliver unique selectivity, rapid equilibration, and exceptional durability.
The Bidentate C8 stationary phase provides moderate hydrophobic retention with alternative selectivity, making it particularly useful when C18 phases retain analytes too strongly. It is ideal for isolating hydrophobic molecules, metabolites, and compounds containing amine functionality, especially when large sample loads must be processed and collected.
The 30 mm internal diameter (ID) places this column firmly in the preparative / purification scale range, allowing significantly higher mass loading compared to analytical columns.
Preparative Column Use‑Case Framework
50 mm Length – Speed & Throughput
Optimized for fast purification cycles
Lower backpressure at high flow rates
Ideal for high‑throughput compound isolation
Preferred when resolution demands are moderate but speed is critical
Flow Rate & System Requirements
30 mm ID Preparative Columns require specialized systems
Typical flow rate range: ~40 – 120 mL/min
Optimal starting range: ~60 – 90 mL/min
Requires:
Preparative HPLC system with high‑flow capabilities
High‑capacity pump heads and tubing (prep‑scale)
Appropriate injection volumes and fraction collection systems
⚠️ Standard analytical HPLC systems are not suitable for this column size.
Flow rates should be optimized based on:
Solvent viscosity
Target resolution
System pressure limits
Sample load requirements
Why Choose Bidentate C8 for Preparative Work
Compared to C18 phases, Bidentate C8:
Provides reduced hydrophobic retention
Enables faster elution of strongly retained compounds
Offers different selectivity for polar and ionizable analytes
Improves recovery of amine‑containing compounds
Reduces cycle time during purification workflows
Cogent TYPE‑C™ Advantages
Silica Hydride Surface Technology
Lower silanol activity vs conventional silica
Improved peak shape for basic analytes
Reduced secondary interactions
Faster Equilibration
Shorter method cycle times
Increased throughput
Robust Column Lifetime
Resistant to hydrolysis and degradation
Stable under demanding prep conditions
Consistent performance over extended use
Typical Applications
Preparative purification of pharmaceuticals
Isolation of metabolites and intermediates
Separation of compounds with amine functionality
High‑load reversed‑phase chromatography
Situations where C18 retention is excessive
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable